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Product Info Summary
| SKU: | PA2140-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Collagen I/COL1A1 Antibody
SKU/Catalog Number
PA2140-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen I/COL1A1 Antibody catalog # PA2140-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen I/COL1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2140-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, identical to the related rat sequence, and different from the related human sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2140-2 is reactive to COL1A1 in Human, Mouse, Rat
Applications
PA2140-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
130 kDa, 220 kDa
Calculated molecular weight
138.941kDa
Background of COL1A1
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence , 5μg/ml, Mouse
Immunofluorescence, 5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat skin tissue lysates,
Lane 3: mouse skin tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.
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Figure 2. IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Collagen I/COL1A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Collagen I/COL1A1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Collagen I/COL1A1 was detected in a paraffin-embedded section of human sophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Collagen I/COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Collagen I/COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)
Gene Name
COL1A1
Full Name
Collagen alpha-1(I) chain
Weight
138.941kDa
Superfamily
fibrillar collagen family
Alternative Names
Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL1A1, check out the COL1A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen I/COL1A1 Antibody (PA2140-2)
Hello CJ!
PA2140-2 has been cited in 96 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone
Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites
Food‐grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood‐testis barrier disruption in mice
Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
PA2140-2 usage in article: APP: WB, SAMPLE:PERITONEAL TISSUE, DILUTION:NA
Li X,Bu X,Yan F,Wang F,Wei D,Yuan J,Zheng W,Su J,Yuan J.Deletion of discoidin domain receptor 2 attenuates renal interstitial fibrosis in a murine unilateral ureteral obstruction model.Ren Fail.2019 Nov;41(1):481-488.doi:10.1080/0886022X.2019.1621759.PMID:31169440;PMCID:PMC6567249.
Species: Mouse
PA2140-2 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Jennifer Bosco,Zhiwei Zhou,Sofie Gabriëls,Mayank Verma,Nan Liu,Brian K. Miller,Sheng Gu,Dianna M. Lundberg,Yan Huang,Eilish Brown,Serene Josiah,Muthuraman Meiyappan,Matthew J. Traylor,Nancy Chen,Atsushi Asakura,Natalie De Jonge,Christophe Blanchetot,Hans de Haard,Heather S. Duffy,Dennis Keefe,VEGFR-1/Flt-1 Inhibition Increases Angiogenesis and Improves Muscle Function in a Mouse Model of Duchenne Muscular Dystrophy,Molecular Therapy - Methods & Clinical Development,2021,,ISSN 2329-0501,https://doi.org/10.1016/j.omtm.2021.03.013.
Species: Human,Mouse,Rat,Monkey
PA2140-2 usage in article: APP:IHC, SAMPLE:DIAPHRAGM, DILUTION:1:1000
Jiang Y,Liu JM,Huang JP,Lu KX,Sun WL,Tan JY,Li BX,Chen LL,Wu Y. Regeneration potential of decellularized periodontal ligament cell sheets combined with 15-Deoxy-Δ12,14-prostaglandin J2nanoparticles in a rat periodontal defect. Biomed Mater.2021 Mar 12.doi:10.1088/1748-605X/abee61.Epub ahead of print.PMID:33711827.
Species: Human,Rat
PA2140-2 usage in article: APP:IHC, SAMPLE:MANDIBLES, DILUTION:1:100
Lombardi, F.; Augello, F.R.; Palumbo, P.; Mollsi, E.; Giuliani, M.; Cimini, A.M.; Cifone, M.G.; Cinque, B. Soluble Fraction from Lysate of a High Concentration Multi-Strain Probiotic Formulation Inhibits TGF-β1-Induced Intestinal Fibrosis on CCD-18Co Cells. Nutrients 2021,13,882. https://doi.org/10.3390/nu13030882
Species: Human
PA2140-2 usage in article: APP:WB, SAMPLE:HUMAN INTESTINAL FIBROBLAST CELL, DILUTION:1:1000
Zhou J,Li R,Liu Q,Zhang J,Huang H,Huang C,Zhang G,Zhao Y,Wu T,Tang Q,Huang Y,Zhang Z,Li Y,He J. Blocking 5-LO pathway alleviates renal fibrosis by inhibiting the epithelial-mesenchymal transition. Biomed Pharmacother. 2021 Mar 12;138:111470.doi:10.1016/j.biopha.2021.111470.Epub ahead of print.PMID:33721755.
Species: Human,Mouse
PA2140-2 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND TCMK-1 CELL, DILUTION:NA
Lan Chen,Xiaofang Ji,Manni Wang et al.Involvement of TLR4 Signaling Regulated-COX2/PGE2 Axis in Liver Fibrosis Induced by Schistosoma japonicum Infection, 06 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs
Species: Human,Mouse
PA2140-2 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:1:400
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Customer Reviews
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Customer Q&As
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20 Customer Q&As for Anti-Collagen I/COL1A1 Antibody
Question
Please see the customer's message. Which antibody do you recommend for their application? "I am conflicted about which primary antibody to order. We are planning to do a Western Blot to measure Collagen Type I in our cell lines (keratinocytes, human mesenchymal stem cells, human dermal fibroblasts cells, and dermal microvascular endothelial cells). I see that the "Anti-Collagen I Rabbit Monoclonal Antibody" detects the Collagen alpha-2 (COL1A2) chain while the other Collagen Type I antibodies you offer detect the Collagen alpha-1 (COL1A1) chain. I am conflicted about which of these two would be best for my studies. I would like advice and more background on the difference between these two and what would be most appropriate for me."
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
There was no obvious difference between COL1A1 and COL1A2 in terms of expression level and location in the samples. Please recommend the customer to use PA2140-2 on the basis of our test result. It is more suitable for working on the inquired samples.
Boster Scientific Support
Answered: 2020-04-29
Question
I have attached the WB image, lot number and protocol we used for fetal brain cortex using anti-Collagen I/COL1A1 antibody PA2140-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-02
Question
Is a blocking peptide available for product anti-Collagen I/COL1A1 antibody (PA2140-2)?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We do provide the blocking peptide for product anti-Collagen I/COL1A1 antibody (PA2140-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-11
Question
We are currently using anti-Collagen I/COL1A1 antibody PA2140-2 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The anti-Collagen I/COL1A1 antibody (PA2140-2) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-06
Question
Will PA2140-2 anti-Collagen I/COL1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
It shows on the product datasheet, PA2140-2 anti-Collagen I/COL1A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
I see that the anti-Collagen I/COL1A1 antibody PA2140-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-Collagen I/COL1A1 antibody PA2140-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-16
Question
We are interested in using your anti-Collagen I/COL1A1 antibody for wound healing studies. Has this antibody been tested with western blotting on placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Thank you for your inquiry. This PA2140-2 anti-Collagen I/COL1A1 antibody is tested on rat lung tissue, tissue lysate, human mammary tissue, placenta tissue, testis tissue, mouse lung. It is guaranteed to work for IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-09
Question
Will anti-Collagen I/COL1A1 antibody PA2140-2 work for WB with fetal brain cortex?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
According to the expression profile of fetal brain cortex, COL1A1 is highly expressed in fetal brain cortex. So, it is likely that anti-Collagen I/COL1A1 antibody PA2140-2 will work for WB with fetal brain cortex.
Boster Scientific Support
Answered: 2019-09-20
Question
A customer wanted to get more information on PA2140-2 as they have been using this antibody for years. Could you assist? "Essentially, what i want to know is if the antibody can recognize the C-terminal telopeptide region (the epitope) only after the C-propeptide of has been cleaved, or if has access to the epitope in the presence of an intact C-propeptide."
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Our lab technicains tested tissue samples which were treated with lysis buffer.
Boster Scientific Support
Answered: 2019-05-21
Question
We purchased anti-Collagen I/COL1A1 antibody for WB on spleen a few years ago. I am using mouse, and I plan to use the antibody for IHC-F next. you antibody examining spleen as well as fetal brain cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-F?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
I have checked the website and datasheets of our anti-Collagen I/COL1A1 antibody and it seems that PA2140-2 has been validated on mouse in both WB and IHC-F. Thus PA2140-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-F in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-F detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-05
Question
I was wanting to use your anti-Collagen I/COL1A1 antibody for WB for rat fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
It shows on the product datasheet, PA2140-2 anti-Collagen I/COL1A1 antibody has been tested for IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-13
Question
I am looking for to test anti-Collagen I/COL1A1 antibody PA2140-2 on rat fetal brain cortex for research purposes, then I may be interested in using anti-Collagen I/COL1A1 antibody PA2140-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
The products we sell, including anti-Collagen I/COL1A1 antibody PA2140-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-19
Question
Do you have a BSA free version of anti-Collagen I/COL1A1 antibody PA2140-2 available?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen I/COL1A1 antibody PA2140-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-15
Question
Our team were well pleased with the WB result of your anti-Collagen I/COL1A1 antibody. However we have observed positive staining in brain secreted using this antibody. Is that expected? Could you tell me where is COL1A1 supposed to be expressed?
G. Krishna
Verified customer
Asked: 2016-01-29
Answer
From literature, brain does express COL1A1. Generally COL1A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2016-01-29
Question
Can PA2140-2 recognize the C-terminal telepeptide region only after the C-propeptide of it has been cleaved, or if it has access to the epitope in the presence of an intact C-propeptide.
Verified Customer
Verified customer
Asked: 2016-01-19
Answer
Our lab technicains tested tissue samples which were treated with lysis buffer. C-propeptide in the protein present in the samples was cleaved. We haven't worked on samples containing protein with an intact C-propeptide. We are uncertain if PA2140-2 would recognize the epitope in the presence of an intact C-propeptide.
Boster Scientific Support
Answered: 2016-01-19
Question
Does anti-Collagen I/COL1A1 antibody PA2140-2 work on monkey WB with spinal cord?
R. Johnson
Verified customer
Asked: 2015-11-09
Answer
Our lab technicians have not validated anti-Collagen I/COL1A1 antibody PA2140-2 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Collagen I/COL1A1 antibody PA2140-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey spinal cord in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-09
Question
My question regarding product PA2140-2, anti-Collagen I/COL1A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Krishna
Verified customer
Asked: 2015-11-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2140-2 anti-Collagen I/COL1A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-11-09
Question
Is this PA2140-2 anti-Collagen I/COL1A1 antibody reactive to the isotypes of COL1A1?
K. Baker
Verified customer
Asked: 2015-02-27
Answer
The immunogen of PA2140-2 anti-Collagen I/COL1A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I(1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-27
Question
We have observed staining in rat spinal cord. Are there any suggestions? Is anti-Collagen I/COL1A1 antibody supposed to stain spinal cord positively?
E. Evans
Verified customer
Asked: 2013-05-01
Answer
From literature spinal cord does express COL1A1. From Uniprot.org, COL1A1 is expressed in spinal cord, spleen, brain, skin, fetal brain cortex, bone, liver, among other tissues. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2013-05-01
